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Microbes
monitor report of Endoscope environment
一、monitor time:
April,2005~August,2005
二、Endoscope washing,
disinfection flow: performed according to <operation
norm of endoscope washing and disinfection>passed by
minister of health in 2004, and the washing process
adopt “kangaking” washing center.
三、Monitor measures:
1、endoscope
body
Sampling: sampling
after sterilization and before use. Embrocate the
endoscope body (30cm below endoscope month) use cotton
cloth which contains sterile eluent, and then turning
the sample cotton cloth, cut away the part which
operator has touched. Put the sample into sterile eluent
test tube which contain relevant neutralization agent,
and submit for censorship instantly.
Monitor measure:
shacking the sample liquid at least 80 times, add 1ml
sample liquid to sterile dish (diameter is 90mm.), and
then add nutrient agar, shacking while injecting, put it
into 35 centigrade incubator for 48 hours and count
after the agar clotted.
(二)、the
inner side of endoscope lumen
Sampling: monitor the
inner side of endoscope lumen. Draw out 10ml buffer
solution which contain corresponding neutralization
agent use sterile injector, and then inject it into
endoscope from active mouth, collect it use 15ml sterile
test tube from active outlet. Submit for censorship in
tow hours.
Colony count: shacking
the sample liquid at least 80 times, add 0.5ml sample
liquid into two sterile dish (diameter is 90mm.), each
of plate add 15ml~18ml melted nutrient (45C~48C),
shacking while injecting, put it into 35 C incubator for
48 hours and count after the agar clotted.
Result judgment :
colony quantity/endoscope=average colony quantity of two
dish×20
Pathogen monitoring:
shacking the sample liquid at least 80 times, and
inoculate 0.2ml to 90mm blood dish, Chinese blue
dish(中国兰平皿)
and SS dish, daubing it equally, then put it into 35C
incubator for 48 hours, observe if there have pathogen
growing.
(三)、monitor
results (look at table 1):
Table 1 the monitor summary of
gastroscope, enteroscope disinfection and sterilization
effect
Monitor item sample quantity
(case) qualification number (case) qualification
rate (%)
Gastroscope
body
Inner side of
Gastroscope lumen
Enteroscope body
Inner side of
Enteroscope lumen
Total
Notice: 1. Pathogen
monitor the inner side of gastroscope and enteroscope,
no pathogen growing.
2. The judge
basis is <operation norm of endoscope washing and
disinfection> (2004), the clause 24.
Sichuan University west china hospital
September, 2005
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